Scanning electron microscopy (SEM) and ICC images suggested the presence of a tissue-like layer with a mixed-phenotype population.
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Additionally, lower neuronal percentages were found on AAO as compared to TCT glass however, neuronal population was also found to vary with pore diameter.
![stony brook pclp stony brook pclp](https://www.stonybrook.edu/commcms/phd-career-ladder/_images/FarzanaAli.png)
Immunocytochemistry (ICC) showed that AAO membranes had increased neurite outgrowth when compared to tissue culture treated (TCT) glass, and neurite outgrowth varied with pore diameter. Analysis of membranes before and after cell culture indicated no breakdown of the through-pore structure. The data suggests a highly tunable correlation between AAO pore diameter and differentiated cell populations. C17.2 neural stem cells were seeded onto the membranes and differentiated via serum withdrawal. An operating window was established for this setup to create membranes with through-pore and disordered pore morphologies. AAO membranes with pore diameters ranging from 34-117 nm were achieved via anodization at a temperature of -1Â☌ in a 2.7% oxalic acid electrolyte. The objective of this study was to characterize the cellular response of AAO membranes with varying pore sizes to serve as a proof-of-concept for an artificial material/cell synapse system.
![stony brook pclp stony brook pclp](https://www.christophertsmith.com/uploads/3/7/4/4/37446679/npa-logo_orig.png)
Anodic Aluminum Oxide ( AAO) Membranes for Cellular DevicesĪnodic Aluminum Oxide ( AAO) membranes can be fabricated with a highly tunable pore structure making them a suitable candidate for cellular hybrid devices with single-molecule selectivity.